Fibrinogen media for studies on staphylococci.

Deneke, Anneliese (University of Wisconsin, Madison) and Hans Blobel. Fibrinogen media for studies on staphylococci. J. Bacteriol. 83:533-537. 1962-Fibrinogen media were prepared simply by spreading rabbit plasma or a bovine fibrinogen solution over the surface of certain solidified agar media, thus eliminating the necessity of incorporation of fibrinogen into liquefied agar media at a critical temperature. With agar media composed of heart infusion broth, blood agar base, or Trypticase soy broth, distinct coagulase reactions were observed which corresponded closely to those obtained with the tube test.A bovine fibrinogen solution, applied to the surface of some of the commonly used selective media for staphylococci, provided an additional and more specific test system for coagulase production of individual colonies, and consequently aided in the presumptive identification of pathogenic staphylococci.